Example Analysis of offline data of Pacbio Sequel

This article will explain in detail the example analysis of Pacbio Sequel offline data. The editor thinks it is very practical, so I share it for you as a reference. I hope you can get something after reading this article.

Introduction to offline data of Pacbio Sequel

Pacbio Sequel outbound data generally includes the following data:

M54134_180724_220656.scraps.bamm54134_180724_220656.subreads.bamm54134_180724_220656.scraps.bam.pbim54134_180724_220656.subreads.bam.pbim54134_180724_220656.baz2bam_1.logm54134_180724_220656.sts.xmlm54134_180724_220656.subreadset.xmlm54134_180724_220656.transferdonem54134_180724_220656.adapters.fasta

The name of the file has a fixed encoding form, take the above data as an example:

M54134_180724_220656.subreads.bam

Where:

M: is the abbreviation of movie

54134: is the number of the equipment

1807240220656: is the start time of the sequencing operation, 22:06:56 on July 24, 2018

Subreads: these are different data types, such as scraps, subreads, etc.

Bam: this is the format of data, such as bam format, xml,fasta format, etc.

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