How to use clusterProfiler in R language to enrich grapes with GO

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The basic process is to compare Hiast2 to bamstringtie assembly transcript gffcompare to compare the gtf file output from stringtie with the annotation file of the reference genome to get a merged.combine.gtf that uses merged.combine.gtf to calculate the expression for each sample, and the output file is stored in the ballgown folder. The command used in this step is stringtie-e-B-p 8-G merged.combined.gtf-o ballgown/L01/L01.gtf output_bam/L01.sorted.bamimage.png, followed by the R language ballgown packet reading data to obtain the gene and transcript expression code is library (ballgown).

Library (genefilter)

Library (dplyr)

Pheno_data