What is the use of LncRNA2Target database
This article mainly introduces the use of LncRNA2Target database, the article is very detailed, has a certain reference value, interested friends must read it!
LncRNA plays an important role in the regulation of gene expression. There are two research strategies to study the target genes regulated by lncRNA. One is to analyze the proteins bound by lncRNA through traditional experimental methods to determine the interaction between lncRNA and protein-coding genes. The common methods are as follows
Immunoprecipitation assays
RNA pull down assays
Luciferase reported assays
Another method is to knock down or over-express a lncRNA, and then detect the differentially expressed genes before and after treatment, and take the differentially expressed genes as the target genes of the lncRNA, either by chip or high-throughput sequencing.
The LncRNA2Target database collates the published lncRNA target gene data, the latest version is v2.0, and the website is as follows
Http://123.59.132.21/lncrna2target/index.jsp
The lncRNA target genes in humans and mice are covered. The corresponding results can be viewed through the Browse menu, as shown below.
Through the Search menu, you can easily retrieve the database, as shown below
The database provides download function and provides lncRNA target gene files analyzed by traditional experiments and high-throughput methods, respectively, corresponding to the following two files
LncRNA_target_from_low_throughput_experiments.xlsx
LncRNA_target_from_high_throughput_experiments.txt
For high-throughput data, the corresponding GEO number, the expression spectrum data and the results of difference analysis are also given, as shown below.
Through this database, we can not only query the target gene information of lncRNA, but also learn the idea of studying lncRNA target gene.
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