How to use ChIPpeakAnno to annotate peak

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ChIPpeakAnno is an R packet on bioconductor. For downstream analysis after peak calling, it provides the following functions

Find the genes closest to the peak region, and also support custom search features, such as exon,miRNA, etc.

GO enrichment Analysis of adjacent genes of peak

Extract the sequence of peak and its surrounding area

In ChIPpeakAnno, both peak interval information and genome annotation information are transformed into GRanges objects in R language by toGRanges method. Take peak as an example, the content of bed format is as follows

You can import this information with the following code

Library (ChIPpeakAnno)

Bed