Shulou(Shulou.com)06/02 Report--

This article shows you how to implement the difference analysis operation between two groups in limma. The content is concise and easy to understand. It will definitely make your eyes shine. I hope you can gain something through the detailed introduction of this article.

1. read the file

Read the gene expression file in all samples, as shown below

gene_id ctrl-1 ctrl-2 ctrl-3 case-1 case-2 case-3geneA 14 0 11 4 0 12geneB 125 401 442 175 59 200

Each row represents a gene, and each column represents a sample. The code for reading the data is as follows

#Read table counts of expression quantities

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